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Browsing by Subject "Molecular biology"

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Determination of diffuse double layer protonation constants for hydrous ferric oxide (HFO): supporting evidence for the Dzombak and Morel compilation

Author: Pretorius, PJ ; Linder, PW Date: 1998 The acid-base properties of hydrous ferric oxide were studied by glass electrode potentiometry. From the potentiometric data, surface protonation constants were derived according to the Diffuse Double Layer convention. Chemical equilibrium ... Read more
A discussion of molecular biology methods for protein engineering

Author: Zawaira, A ; Pooran, A ; Barichievy, S ; Chopera, D Date: Sep 2011 A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve ... Read more
Establishing malaria parasite transfection technology in South Africa

Author: Van Brummelen, AC ; Becker, JVW ; Mancama, Dalubuhle T ; Hoppe, HC Date: Jan 2010 The most important contributing factor to the current malaria crisis is the rapid spread of parasite resistance to available anti-malarial drugs. Anti-malarial drug resistance is critical and the need for compounds with novel modes of action ... Read more
Establishing malaria parasite transfection technology in South Africa.

Author: Van Brummelen, AC ; Becker, JVW ; Mancama, Dalubuhle T ; Hoppe, H Date: Jan 2010 In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), ... Read more
Exploring viral diversity in a unique South African soil habitat

Author: Segobola, J ; Adriaenssens, E ; Tsekoa, Tsepo L ; Rashamuse, Konanani ; Cowan, D Date: Jan 2018 The Kogelberg Biosphere Reserve in the Cape Floral Kingdom in South Africa is known for its unique plant biodiversity. The potential presence of unique microbial and viral biodiversity associated with this unique plant biodiversity led us to ... Read more
Immobilisation and characterisation of glucose dehydrogenase immobilised on ReSyn: a proprietary polyethylenimine support matrix

Author: Twala, BV ; Sewell, BT ; Jordaan, J Date: Jan 2010 Immobilisation of enzymes is of considerable interest due to the advantages over soluble enzymes, including improved stability and recovery. Glucose Dehydrogenase (GDH) is an important biocatalytic enzyme due to is ability to recycle the ... Read more
Paper based microfluidic devices for environmental diagnostics

Author: Govindasamy, K ; Potgieter, S ; Kumar, S ; Land, K ; Muzenda, E Date: Sep 2012 Microfluidics has found widespread application in the fields of molecular biology, DNA analysis and most recently, point of care diagnostics. We present a paper based microfluidic device for rapid, in-the-field detection of pathogenic bacteria ... Read more

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Browsing by Subject "Molecular biology"

Browsing by Subject "Molecular biology"

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