ResearchSpace
→
Browsing by Author

Browsing by Author "Mathiba, K"

Now showing items 1-10 of 10

Application of biocatalysis in synthetic chemistry

Author: Brady, D ; Molefe, M ; Steenkamp, Lucia H ; Parkinson, C ; Jordaan, J ; Visser, Daniel F ; Simpson, C ; Chhiba, Varsha P ; Mathiba, K Date: Nov 2006 It was found that the nitrile hydratases in microorganisms isolated from around the world had infra-species amino acid sequence differences, and this provided an explanation for the variability of nitrile substrate usage within the species. ... Read more
Cell free phosphorylation method to assess the utility of new nucleotides as nucleoside reserve transcriptase inhibitors

Author: Lebea, Phiyani J ; Bode, ML ; Mathiba, K ; Brady, D Date: Jun 2007 The acyclic nucleoside phosphonates such as cidofovir, adefovir and tenofovir have proved to be effective in vitro (cell culture systems) and in vivo (animal models, clinical studies) against a wide variety of DNA virus and retrovirus infections. ... Read more
Cloning, multicopy expression and fed-batch production of Rhodotorula araucariae epoxide hydrolase in yarrowia lipolytica

Author: Ramduth, D ; Roth, Robyn L ; Lalloo, Rajesh ; Simpson, C ; Mitra, RK ; Gorgens, J ; Ramchuran, Santosh O ; Mathiba, K Date: May 2008 Epoxide hydrolases (EHs) of fungal origin have the ability to catalyse the enantioselective hydrolysis of epoxides to their corresponding diols. However wild type fungal EHs are limited in the substrate range and enantioselectivity, additionally ... Read more
Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870

Author: Chhiba, Varsha P ; Bode, M ; Mathiba, K ; Kwezi, Lusisizwe ; Brady, D Date: Apr 2012 A range of ß-aminonitriles (3-amino-3-phenylpropanenitrile and derivatives) were synthesised by reaction of various benzonitriles with acetonitrile and subsequent reduction of the resulting acrylonitrile products. These compounds were hydrolysed ... Read more
Enzymatic stereoselective synthesis of B-amino acids

Author: Chhiba, Varsha P ; Bode, M ; Mathiba, K ; Brady, D Date: Jun 2014 The use of enzymes for the enantioselective synthesis of single enantiomer ß-subsituted compounds is of interest, as this structural motif occurs commonly in compounds of pharmaceutical importance, such as adrenergic agents, antidiabetics, ... Read more
Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine

Author: Visser, Daniel F ; Gordon, GER ; Bode, ML ; Mathiba, K ; Brady, D Date: May 2012 5-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of ... Read more
Functional characterisation of a metagenome derived family VIII esterase with a deacetylation activity on ß-lactam antibiotics

Author: Mokoena, N ; Mathiba, K ; Tsekoa, Tsepo L ; Steenkamp, P ; Rashamuse, K Date: Aug 2013 Family VIII esterases represent a poorly characterised esterase family, with high sequence identity to class C b-lactamases, peptidases and penicillin binding protein. In this study we report on the metagenomic screening and biochemical ... Read more
High yielding cascade enzymatic synthesis of 5-methyluridine using a novel Purine Nucleoside Phosphorylase, from Bacillus halodurans Alk36

Author: Vissser, D ; Hennessy, F ; Rashamuse, K ; Van Zyl, P ; Gordon, G ; Mathiba, K ; Bode, M ; Pletschke, B ; Brady, D Date: Sep 2010 5-Methyluridine(5-MU) is a non-natural nucleoside that can be used as an intermediate in the synthesis of thymidine, and in the synthesis of nucleoside analogues AZT and stavudine, both of which are used in Highly Active Anti-Retroviral ... Read more
Metagenomic mining of feruloyl esterases from termite enteric flora

Author: Rashamuse, K ; Ronneburg, T ; Sanyika, W ; Mathiba, K ; Mmutlane, E ; Brady, D Date: Jan 2014 A metagenome expression library was created from Trinervitermes trinervoides termite hindgut symbionts and subsequently screened for feruloyl esterase (FAE) activities, resulting in seven recombinant fosmids conferring feruloyl esterase ... Read more

Now showing items 1-10 of 10

Browse

All of ResearchSpace

Quick Links

Legislation and compliance

General Enquiries

Tel: + 27 12 841 2911
Email: callcentre@csir.co.za

Physical Address
Meiring Naudé Road
Brummeria
Pretoria
South Africa

Postal Address
PO Box 395
Pretoria 0001
South Africa

Social Connect

Resources on this site are free to download and reuse according to associated licensing provision. Please read the terms and conditions of usage of each resource.

Browsing by Author "Mathiba, K"

Browsing by Author "Mathiba, K"

Browse

All of ResearchSpace

Quick Links

Legislation and compliance

General Enquiries

Social Connect