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Biochemical pulldown of mRNAs and long noncoding RNAs from cellular lysates coupled with mass spectrometry to identify protein binding partners

Author: Savulescu, AF ; Stoychev, Stoyan H ; Mamputha, Sipho ; Mhlanga, MM Date: Jun 2020 RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition ... Read more
Challenges and opportunities for biological mass spectrometry core facilities in the developing world

Author: Bell, L ; Calder, B ; Hiller, R ; Klein, A ; Soares, NC ; Stoychev, Stoyan H ; Vorster, BC ; Tabb, DL Date: Apr 2018 The developing world is seeing rapid growth in the availability of biological mass spectrometry (MS), particularly through core facilities. As proteomics and metabolomics becomes locally feasible for investigators in these nations, application ... Read more
Evaluation of novel supports for selective and efficient enrichment of phosphorylated peptides

Author: Bensadek, D ; Chen, W ; Jordaan, J ; Gerber, I ; Lamont, D Date: Jun 2011 A series of novel emulsion based supports MagReSynTM TiO2 (A-D) were evaluated using 32P labelled phosphopeptides and nano LC-MS/MS. The performance of MagReSynTM TiO2 (D) was found to be significantly better in terms of selectivity and ... Read more
Identification of atractyloside by LC-ESI-MS in alleged herbal poisoning

Author: Steenkamp, PA ; Harding, NM ; Van Heerden, FR ; Van Wyk, BE Date: Nov 2006 An LC-MS screening method was developed to detect the presence of atractyloside (ATR), the toxic principle of a commonly used medicinal plant in South Africa, Callilepis laureola, in biological matrices such as body fluids and human viscera. ... Read more
Phosphorylation- and nucleotide-binding-induced changes to the stability and hydrogen exchange patterns of JNK1ß1 provide insight into its mechanisms of activation

Author: Owen, GR ; Stoychev, Stoyan H ; Achilonu, I ; Dirr, HW Date: Oct 2014 Many studies have characterized how changes to the stability and internal motions of a protein during activation can contribute to their catalytic function, even when structural changes cannot be observed. Here, unfolding studies and ... Read more
Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling

Author: Koenig, C ; Martinez-Val, A ; Naicker, Previn ; Stoychev, S ; Jordaan, J ; Olsen, JV Date: Sep 2023 Tandem mass tags data-dependent acquisition (TMT-DDA) as well as data-independent acquisition-based label-free quantification (LFQ-DIA) have become the leading workflows to achieve deep proteome and phosphoproteome profiles. We present a ... Read more
Redox exchange of the disulfides of human two-domain CD4 regulates the conformational dynamics of each domain, providing insight into its mechanisms of control

Author: Owen, GR ; Le, D ; Stoychev, Stoyan H ; Cerutti, NM ; Papathanasopoulos, M Date: Mar 2018 CD4, a membrane glycoprotein expressed by specific leukocytes, plays a vital role in the human immune response and acts as a primary receptor for HIV entry. Of its four ecto-domains (D1-D4), D1, D2, and D4 each contain a distinctive disulfide ... Read more
Zirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics

Author: Diez, IA ; Govender, Ireshyn S ; Naicker, Previn ; Stoychev, Stoyan H ; Jordaan, J ; Jensen, ON Date: Apr 2020 Phosphopeptide enrichment is an essential step in large-scale, quantitative phosphoproteomics studies by mass spectrometry. Several phosphopeptide affinity enrichment techniques exist, such as Immobilized Metal ion Affinity Chromatography ... Read more

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Browsing by Subject "Mass spectrometry"

Browsing by Subject "Mass spectrometry"

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