Author:Visser, Daniel F; Hennessy, F; Rashamuse, KJ; Louw, ME; Brady, DDate:Mar 2010A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ...Read more