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Browsing by Subject "Epoxide hydrolases"

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Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1, 2-epoxyoctane

Author: Maritz, J ; Krieg, HM ; Yeates, CA ; Botes, AL ; Breytenbach, JC Date: Oct 2003 Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilized in calcium alginate beads for the enantio selective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was ... Read more
Cloning of an epoxide hydrolase encoding gene from Rhodotorula mucilaginosa and functional expresion in Yarrowia lipolytica

Author: Labuschagne, M ; Albertyn, J Date: Jan 2007 Epoxide hydrolases (EHs), especially those of fungal origin, have the ability to catalyse the enantioselective hydrolysis of epoxides to their corresponding diols. Recombinant DNA technology has been used extensively to overproduce these ... Read more
Cloning, multicopy expression and fed-batch production of Rhodotorula araucariae epoxide hydrolase in yarrowia lipolytica

Author: Ramduth, D ; Roth, Robyn L ; Lalloo, Rajesh ; Simpson, C ; Mitra, RK ; Gorgens, J ; Ramchuran, Santosh O ; Mathiba, K Date: May 2008 Epoxide hydrolases (EHs) of fungal origin have the ability to catalyse the enantioselective hydrolysis of epoxides to their corresponding diols. However wild type fungal EHs are limited in the substrate range and enantioselectivity, additionally ... Read more
Correlation between the physicochemical properties of organic solvents and their biocompatibility toward epoxide hydrolase activity in whole-cells of a yeast, Rhodotorulasp

Author: Lotter, J ; Botes, AL ; Van Dyk, MS ; Breytenbach, JC Date: Aug 2004 Epoxides are often highly hydrophobic substrates and the presence of an organic co-solvent within an aqueous bioreactor is in such cases indicated. The effect of 40 water-miscible and -immiscible organic solvents on epoxide hydrolase activity ... Read more
Hydrolytic kinetic resolution of the enantiomers of the structural isomers trans-1-phenylpropene oxide and (2,3-epoxypropyl) benzene by yeast epoxide hydrolase

Author: Lotter, J ; Botes, AL ; Dyk, MS ; Breytenbach, JC Date: Aug 2004 Kinetic resolution of the enantiomers of trans-1-phenylpropene oxide and (2,3-epoxypropyl)benzene was achieved by yeasts from the genus Rhodotorula. The resolution of trans-1-phenylpropene oxide by Rhodotorula glutinis UOFS Y-0123 yielded ... Read more
Interspecies differences in the enantioselectivity of epoxide hydrolases in Cryptococcus laurentii (Kufferath) C.E. Skinner and Cryptococcus podzolicus (Bab'jeva & Reshetova) Golubev

Author: Botes, AL ; Lotter, J ; Rhode, OHJ ; Botha, A Date: Jan 2005 Isolates representing Cryptococcus laurentii and Cryptococcus podzolicus, originating from soil of a heath land indigenous to South Africa, were screened for the presence of enantioselective epoxide hydrolases for 2, 2-disubstituted epoxides. ... Read more

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Browsing by Subject "Epoxide hydrolases"

Browsing by Subject "Epoxide hydrolases"

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