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Browsing by Subject "Bacillus halodurans"

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Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
Development of a flagellin surface display expression system in a moderate thermophile, Bacillus halodurans Alk36

Author: Crampton, Michael C ; Berger, E ; Reid, S ; Louw, M Date: Jun 2007 This study relates to the development of an alkaliphilic, thermotolerant, Gram-positive isolate, Bacillus halodurans Alk36, for the over-production and surface display of chimeric gene products. This bacterium continuously over-produces ... Read more
Evaluation of staphylococcus aureus class C non-specific acid phosphatase (SapS) as a reporter for gene expression and protein secretion in gram-negative and gram-positive bacteria

Author: Du Plessis, EM ; Theron, J ; Berger, E ; Louw, ME Date: Nov 2007 A phosphatase secreted by Staphylococcus aureus strain 154 has previously been characterized and classified as a new member of the bacterial class C family of non-specific acid phosphatases. As the acid phosphatase activity can be easily ... Read more
Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system

Author: Berger, E ; Crampton, Michael C ; Nxumalo, NP ; Louw, ME Date: Aug 2011 Through modification of the flagellin type III secretion pathway of Bacillus halodurans heterologous peptides could be secreted into the medium as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced ... Read more
Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression

Author: Berger, E ; Du Plessis, E ; Gerber, I ; Crampton, Michael C ; Nxumalo, NP ; Louw, M Date: Jan 2009 The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as ... Read more
Production of a lipolytic enzyme originating from Bacillus halodurans LBB2 in the methylogrophic yeast Pichia pastoria

Author: Ramchuran, Santosh O Date: Jul 2006 A gene encoding a lipolytic enzyme amplified from the alkaliphilic bacterium Bacillus halodurans LBB2 was cloned into the pPICZaB vector and integrated into the genome of the protease deficient yeast strain Pichia pastoris SMD1168H. This ... Read more
Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans

Author: Crampton, Michael C Date: Jul 2010 This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation ... Read more

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Browsing by Subject "Bacillus halodurans"

Browsing by Subject "Bacillus halodurans"

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