Author:Theron, J; Cilliers, Jacques E; Du Preez, M; Brozel, VS; Venter, SNDate:Sep 2000A pit-stop semi-nested PCR assay for the detection of toxigenic Vibrio cholerae in environmental water samples was developed and its performance evaluated. The PCR technique amplifies sequences within the cholera toxin operon specific for ...Read more
Author:Ssemakalu, CC; Ubomba-Jaswa, Eunice; Motaung, KS; Pillay, MDate:Jun 2015The use of solar irradiation to sterilize water prior to its consumption has resulted in the reduction of water related illnesses in waterborne disease endemic communities worldwide. Currently, research on solar water disinfection (SODIS) has ...Read more
Author:Le Roux, Wouter J; Chan, WY; De Maayer, P; Venter, SNDate:Mar 2013Vibrio cholerae, a Gram-negative pathogen autochthonous to the aquatic environment, is the causative agent of cholera. Here, we report the complete genome sequence of V. cholerae G4222, a clinical isolate from South Africa.Read more
Author:Ssemakalu, CC; Le Roux, Wouter J; Pillay, MDate:Sep 2013Cholera toxin is the aetiological agent of cholera – a deadly waterborne disease acquired through the consumption of untreated water contaminated with CTX bacteriophage harbouring strains of V. cholerae. Solar disinfection is a re-emerging ...Read more
Author:Woodborne, S; Pienaar, M; Van der Merwe, Martina RDate:Nov 2008Cholera epidemics have been analysed using epidemiological models that depend on human-to environment- to-human spread of toxigenic Vibrio cholerae bacteria (Koelle et al. 2004, Koelle et al. 2005). Within such a framework the vulnerable human ...Read more
Author:Le Roux, Wouter J; Van Blerk, GNDate:Oct 2011A real-time polymerase chain reaction (PCR) assay utilizing high resolution melt (HRM) curve analysis was developed and tested for the monitoring of Vibrio cholerae in water samples. The assay utilized previously published primers that are ...Read more