Author:Savulescu, AF; Stoychev, Stoyan H; Mamputha, Sipho; Mhlanga, MMDate:Jun 2020RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition ...Read more
Author:Bell, L; Calder, B; Hiller, R; Klein, A; Soares, NC; Stoychev, Stoyan H; Vorster, BC; Tabb, DLDate:Apr 2018The developing world is seeing rapid growth in the availability of biological mass spectrometry (MS), particularly through core facilities. As proteomics and metabolomics becomes locally feasible for investigators in these nations, application ...Read more
Author:Bensadek, D; Chen, W; Jordaan, J; Gerber, I; Lamont, DDate:Jun 2011A series of novel emulsion based supports MagReSynTM TiO2 (A-D) were evaluated using 32P labelled phosphopeptides and nano LC-MS/MS. The performance of MagReSynTM TiO2 (D) was found to be significantly better in terms of selectivity and ...Read more
Author:Steenkamp, PA; Harding, NM; Van Heerden, FR; Van Wyk, BEDate:Nov 2006An LC-MS screening method was developed to detect the presence of atractyloside (ATR), the toxic principle of a commonly used medicinal plant in South Africa, Callilepis laureola, in biological matrices such as body fluids and human viscera. ...Read more
Author:Owen, GR; Stoychev, Stoyan H; Achilonu, I; Dirr, HWDate:Oct 2014Many studies have characterized how changes to the stability and internal motions of a protein during activation can contribute to their catalytic function, even when structural changes cannot be observed. Here, unfolding studies and ...Read more
Author:Koenig, C; Martinez-Val, A; Naicker, Previn; Stoychev, S; Jordaan, J; Olsen, JVDate:Sep 2023Tandem mass tags data-dependent acquisition (TMT-DDA) as well as data-independent acquisition-based label-free quantification (LFQ-DIA) have become the leading workflows to achieve deep proteome and phosphoproteome profiles. We present a ...Read more
Author:Owen, GR; Le, D; Stoychev, Stoyan H; Cerutti, NM; Papathanasopoulos, MDate:Mar 2018CD4, a membrane glycoprotein expressed by specific leukocytes, plays a vital role in the human immune response and acts as a primary receptor for HIV entry. Of its four ecto-domains (D1-D4), D1, D2, and D4 each contain a distinctive disulfide ...Read more
Author:Diez, IA; Govender, Ireshyn S; Naicker, Previn; Stoychev, Stoyan H; Jordaan, J; Jensen, ONDate:Apr 2020Phosphopeptide enrichment is an essential step in large-scale, quantitative phosphoproteomics studies by mass spectrometry. Several phosphopeptide affinity enrichment techniques exist, such as Immobilized Metal ion Affinity Chromatography ...Read more