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Browsing by Subject "Guanosine"

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Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine

Author: Visser, Daniel F ; Gordon, GER ; Bode, ML ; Mathiba, K ; Brady, D Date: May 2012 5-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of ... Read more

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Browsing by Subject "Guanosine"

Browsing by Subject "Guanosine"

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