Author:Maritz, J; Krieg, HM; Yeates, CA; Botes, AL; Breytenbach, JCDate:Oct 2003Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilized in calcium alginate beads for the enantio selective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was ...Read more
Author:Labuschagne, M; Albertyn, JDate:Jan 2007Epoxide hydrolases (EHs), especially those of fungal origin, have the ability to catalyse the enantioselective hydrolysis of epoxides to their corresponding diols. Recombinant DNA technology has been used extensively to overproduce these ...Read more
Author:Lotter, J; Botes, AL; Van Dyk, MS; Breytenbach, JCDate:Aug 2004Epoxides are often highly hydrophobic substrates and the presence of an organic co-solvent within an aqueous bioreactor is in such cases indicated. The effect of 40 water-miscible and -immiscible organic solvents on epoxide hydrolase activity ...Read more
Author:Lotter, J; Botes, AL; Dyk, MS; Breytenbach, JCDate:Aug 2004Kinetic resolution of the enantiomers of trans-1-phenylpropene oxide and (2,3-epoxypropyl)benzene was achieved by yeasts from the genus Rhodotorula. The resolution of trans-1-phenylpropene oxide by Rhodotorula glutinis UOFS Y-0123 yielded ...Read more
Author:Botes, AL; Lotter, J; Rhode, OHJ; Botha, ADate:Jan 2005Isolates representing Cryptococcus laurentii and Cryptococcus podzolicus, originating from soil of a heath land indigenous to South Africa, were screened for the presence of enantioselective epoxide hydrolases for 2, 2-disubstituted epoxides. ...Read more