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Browsing by Subject "Directed evolution"

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Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization

Author: Visser, Daniel F ; Hennessy F ; Rashamuse J ; Pletschke B ; Brady D Date: Aug 2010 Uridine phosphorylase from Escherichia coli was evolved by iterative saturation mutagenesis. The best mutant showed a temperature optimum of 60C and a half-life of 17.3 h at 60C. The mutant enzyme, as well as a purine nucleoside phosphorylase ... Read more

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Browsing by Subject "Directed evolution"

Browsing by Subject "Directed evolution"

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