Author:Visser, Daniel F; Gordon, GER; Bode, ML; Mathiba, K; Brady, DDate:May 20125-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of ...Read more
Author:Visser, Daniel F; Hennessy F; Rashamuse J; Pletschke B; Brady DDate:Aug 2010Uridine phosphorylase from Escherichia coli was evolved by iterative saturation mutagenesis. The best mutant showed a temperature optimum of 60C and a half-life of 17.3 h at 60C. The mutant enzyme, as well as a purine nucleoside phosphorylase ...Read more