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Browsing by Subject "5-Methyluridine"

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Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
High yielding cascade enzymatic synthesis of 5-methyluridine using a novel Purine Nucleoside Phosphorylase, from Bacillus halodurans Alk36

Author: Vissser, D ; Hennessy, F ; Rashamuse, K ; Van Zyl, P ; Gordon, G ; Mathiba, K ; Bode, M ; Pletschke, B ; Brady, D Date: Sep 2010 5-Methyluridine(5-MU) is a non-natural nucleoside that can be used as an intermediate in the synthesis of thymidine, and in the synthesis of nucleoside analogues AZT and stavudine, both of which are used in Highly Active Anti-Retroviral ... Read more

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Browsing by Subject "5-Methyluridine"

Browsing by Subject "5-Methyluridine"

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