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Browsing by Author "Tsekoa, Tsepo L"

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Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4

Author: Makungo, T ; Tsekoa, Tsepo L ; Theron, Anjo ; Mancama, Dalubuhle T ; Van Ree, T Date: Apr 2019 Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4: The increasing incidence of Plasmodium strains that are resistant to current frontline antimalarial drugs has become one of the greatest challenges of ... Read more
Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4

Author: Makungo, T ; Tsekoa, Tsepo L ; Theron, Anjo ; Mancama, Dalubuhle T ; Van Ree, T Date: Apr 2019 Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4: The increasing incidence of Plasmodium strains that are resistant to current frontline antimalarial drugs has become one of the greatest challenges of ... Read more
Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197

Author: Roth, Robyn L ; Van Zyl, Petrus J ; Tsekoa, Tsepo L ; Stoychev, Stoyan H ; Mamputha, Sipho ; Buthelezi, Sindisiwe G ; Crampton, Michael C Date: Apr 2017 The aim of this article is to investigate the production of soluble cross-reacting material 197 (CRM(sub197)) in Escherichia coli, a safe and effective T-cell-dependent protein carrier for polysaccharides used in the manufacture and application ... Read more
Efficient in vitro and in vivo activity of glyco-engineered plant-produced rabies monoclonal antibodies E559 and 62-71-3

Author: Tsekoa, Tsepo L ; Lotter-Stark, Therese ; Buthelezi, Sindisiwe G ; Chakauya, Ereck ; Stoychev, Stoyan H ; Chikwamba, Rachel K Date: Jul 2016 Rabies is a neglected zoonotic disease that has no effective treatment after onset of illness. However the disease can be prevented effectively by prompt administration of post exposure prophylaxis which includes administration of passive ... Read more c b
Enabling local production of biopharmaceuticals in South Africa

Author: Tsekoa, Tsepo L Date: Oct 2017 This presentation discusses the CSIR biopharmaceutical platforms in response to national health priorities and strategic context. Several CSIR-engineered biopharmaceutical case studies are highlighted. Read more
Engineering approaches in plant molecular farming for global health

Author: Singh, Advaita A ; Pillay, Priyen ; Tsekoa, Tsepo L Date: Nov 2021 Since the demonstration of the first plant-produced proteins of medical interest, there has been significant growth and interest in the field of plant molecular farming, with plants now being considered a viable production platform for vaccines. ... Read more
Exploring viral diversity in a unique South African soil habitat

Author: Segobola, J ; Adriaenssens, E ; Tsekoa, Tsepo L ; Rashamuse, Konanani ; Cowan, D Date: Jan 2018 The Kogelberg Biosphere Reserve in the Cape Floral Kingdom in South Africa is known for its unique plant biodiversity. The potential presence of unique microbial and viral biodiversity associated with this unique plant biodiversity led us to ... Read more
Functional characterisation of a metagenome derived family VIII esterase with a deacetylation activity on ß-lactam antibiotics

Author: Mokoena, N ; Mathiba, K ; Tsekoa, Tsepo L ; Steenkamp, P ; Rashamuse, K Date: Aug 2013 Family VIII esterases represent a poorly characterised esterase family, with high sequence identity to class C b-lactamases, peptidases and penicillin binding protein. In this study we report on the metagenomic screening and biochemical ... Read more
Gel and gel-free approaches for the quantitative characterisation of complex protein mixtures

Author: Buthelezi, Sindisiwe G ; Tsekoa, Tsepo L ; Stoychev, Stoyan H ; Mancama, Dalubuhle T ; Blackburn, J Date: Oct 2012 The research aims to establish a reliable set of methods for profiling proteins in a complex mixture in order to allow for the mining of low abundant species. Read more
Molecular modelling of calcium dependent protein kinase 4 (CDPK4) from Plasmodium falciparum

Author: Tsekoa, Tsepo L Date: Oct 2009 Malaria continues to be one of the most serious global health challenges. The increasing incidence of drug resistant Plasmodium strains has emphasised the need for urgent action in the development of new therapeutic strategies against this ... Read more
Molecular Modelling of Calcium Dependent Protein Kinase 4 (CDPK4) from Plasmodium falciparum

Author: Tsekoa, Tsepo L ; Makungo, T ; Mancama, Dalubuhle T Date: Jul 2012 Malaria continues to be one of the most serious global health challenges. The increasing incidence of drug resistant Plasmodium strains has emphasised the need for urgent action in the development of new therapeutic strategies against this ... Read more
Plant-based production of highly potent anti-HIV antibodies with engineered posttranslational modifications

Author: Singh, Advaita Acarya ; Pooe, O ; Kwezi, Lusisizwe ; Lotter-Stark, T ; Stoychev, Stoyan H ; Alexandra, Kabamba B ; Gerber, Isak B ; Bhiman, JN ; Vorster, J ; Pauly, M ; Zeitlin, L ; Whaley, K ; Mach, L ; Steinkellner, H ; Morris, L ; Tsekoa, Tsepo L ; Chikwamba, Rachel K Date: Apr 2020 Broadly neutralising antibodies (bNAbs) against human immunodeficiency virus type 1 (HIV-1), such as CAP256-VRC26 are being developed for HIV prevention and treatment. These Abs carry a unique but crucial post-translational modification (PTM), ... Read more
A plant-biotechnology approach for producing highly potent anti-HIV antibodies for antiretroviral therapy consideration

Author: Singh, Advaita A ; Pillay, Priyen ; Kwezi, Lusisizwe ; Tsekoa, Tsepo L Date: Dec 2021 Despite a reduction in global HIV prevalence the development of a pipeline of new therapeutics or pre-exposure prophylaxis to control the HIV/AIDS epidemic are of high priority. Antibody-based therapies offer several advantages and have been ... Read more
Plant-produced Bluetongue chimaeric VLP vaccine candidates elicit serotype-specific immunity in sheep

Author: Mokoena, NB ; Moetlhoa, B ; Rutkowska, Daria A ; Mamputha, Sipho ; Dibakwane, VS ; Tsekoa, Tsepo L ; O’Kennedy, Martha M Date: Sep 2019 Bluetongue (BT) is a hemorrhagic non-contagious, biting midge-transmitted disease of wild and domestic ruminants that is caused by bluetongue virus (BTV). Annual vaccination plays a pivotal role in BT disease control in endemic regions. Due ... Read more
Recombinant expression, purification and PEGylation of DNA Ligases

Author: Zuma, LK ; Gasa, NL ; Mazibuko, X ; Simelane, BC ; Pillay, Priyen ; Kwezi, Lusisizwe ; Tsekoa, Tsepo L ; Pooe, OJ Date: Apr 2022 Background: Reagent proteins such as DNA ligases play a central role in the global reagents market. DNA ligases are routinely used and are vital in academic and science research environments. Their major functions include sealing nicks by ... Read more
Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses

Author: Dennis, SJ ; O'Kennedy, Maretha M ; Rutkowska, Daria A ; Tsekoa, Tsepo L ; Lourens, Carina W ; Hitzeroth, II ; Meyers, AE ; Rybicki, EP Date: Oct 2018 African horse sickness (AHS) is caused by multiple serotypes of the dsRNA AHSV and is a major scourge of domestic equids in Africa. While there are well established commercial live attenuated vaccines produced in South Africa, risks associated ... Read more
The study of degradation mechanisms of glyco-engineered plant produced anti-rabies monoclonal antibodies E559 and 62-71-3

Author: Buthelezi, Sindisiwe G ; Dirr, HW ; Chakauya, Ereck ; Chikwamba, Rachel K ; Martens, L ; Tsekoa, Tsepo L ; Vandermarliere, E ; Stoychev, Stoyan H Date: Dec 2018 Rabies is an ancient and neglected zoonotic disease caused by the rabies virus, a neurotropic RNA virus that belongs to the Rhabdoviridae family, genus Lyssavirus. It remains an important public health problem as there are cost and health ... Read more
Transient proteolysis reduction of Nicotiana benthamiana-produced CAP256 broadly neutralizing antibodies using CRISPR/Cas9

Author: Singh, Advaita A ; Pillay, Priyen ; Naicker, Previn ; Alexandre, Kabamba B ; Malatji, Kanyane ; Mach, L ; Steinkellner, H ; Vorster, J ; Chikwamba, Rachel K ; Tsekoa, Tsepo L Date: Aug 2022 The hypersensitive response is elicited by Agrobacterium infiltration of Nicotiana benthamiana, including the induction and accumulation of pathogenesis-related proteins, such as proteases. This includes the induction of the expression of ... Read more

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