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Browsing by Author "Chikwamba, Rachel K"

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Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site

Author: Alexandre, Kabamba B ; Gray, ES ; Pantophlet, R ; Moore, PL ; McMahon, JB ; Chakauya, E ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Sep 2011 The glycans on HIV-1 gp120 play an important role in shielding neutralization sensitive epitopes from antibody recognition. They also serve as targets for lectins that bind mannose-rich glycans. In this study we investigated the interaction ... Read more
Binding of the mannose-specific lectin, Griffithsin, to HIV-1 gp120 exposes the CD4-binding site

Author: Alexandre, Kabamba B ; Gray, ES ; Pantophlet, R ; Moore, PL ; McMahon, JB ; Chakauya, E ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Sep 2011 The glycans on HIV-1 gp120 play an important role in shielding neutralization-sensitive epitopes from antibody recognition. They also serve as targets for lectins that bind mannose-rich glycans. In this study, the authors investigated the ... Read more
Biolistic mediated sorghum (Sorghum bicolor L. Moench) transformation via mannose and bialaphos based selection systems

Author: Grootboom, AW ; Mkhonza, NL ; O'Kennedy, Maretha M ; Chakauya, E ; Kunert, K ; Chikwamba, Rachel K Date: 2010 Sorghum (Sorghum bicolor (L.) Moench) is recalcitrant to transformation and besides several reported cases of success, transformation is far from routine. The choice of the selectable markers is key factor influencing success and efficiency ... Read more
Co-suppression of synthesis of major x-kafirin sub-class together with y-kafirin-1 and y-kafirin-2 required for substantially improved protein digestibility in transgenic sorghum

Author: Grootboom, AW ; Mkhonza, NL ; Mbambo, Z ; O'Kennedy, Maretha M ; Da Silva, LS ; Taylor, J ; Taylor, JRN ; Chikwamba, Rachel K ; Mehlo, L Date: Jan 2014 Co-suppressing major kafirin sub-classes is fundamental to improved protein digestibility and nutritional value of sorghum. The improvement is linked to an irregularly invaginated phenotype of protein bodies. Read more
Effect of expressing an anti-HIV lectin, Griffithsin, in different plant cellular compartments

Author: Stark, T ; Chakauya, E ; Kabamba, A ; Morris, L ; Rybicki, E ; Chikwamba, Rachel K Date: Aug 2010 Sub-Saharan Africa remains the region most heavily affected by HIV, and the majority of new infections from heterosexual contact are in women. Griffithsin (GRFT), a lectin isolated from the red algae Griffithsia, has shown extreme potency ... Read more
Effect of subcellular targeting on the expression and accumulation of Griffithsin in N. benthamiana

Author: Stark, T ; Chakauya, E ; Kabamba, A ; Morris, L ; Rybicki, E ; Chikwamba, Rachel K Date: 01 Sep 2010 Due to the increasing demand for pharmaceuticals and the high cost of production in traditional fermentor systems, plants are being considered as alternative production platforms for therapeutic molecules. One of the considerations for ... Read more
Efficient in vitro and in vivo activity of glyco-engineered plant-produced rabies monoclonal antibodies E559 and 62-71-3

Author: Tsekoa, Tsepo L ; Lotter-Stark, Therese ; Buthelezi, Sindisiwe G ; Chakauya, Ereck ; Stoychev, Stoyan H ; Chikwamba, Rachel K Date: Jul 2016 Rabies is a neglected zoonotic disease that has no effective treatment after onset of illness. However the disease can be prevented effectively by prompt administration of post exposure prophylaxis which includes administration of passive ... Read more c b
Elicitor and fusarium-induced expression of NPR-1 like genes in banana

Author: Endah, R ; Beyene, G ; Kiggundu, A ; Van den Berg, N ; Schlüter, U ; Kunert, K ; Chikwamba, Rachel K Date: Nov 2008 NPR1 is an essential positive regulator of salicylic acid-induced PR gene expression and systemic acquired resistance. Two novel full-length NPR1-like genes; MNPR1A and MNPR1B, were isolated by application of the PCR and RACE techniques. The ... Read more
Expression of affordable microbicides to combat the spread of HIV pandemic

Author: Mawela, K ; Lezar, S ; Chakauya, E ; Eloff, JN ; Chikwamba, Rachel K Date: 01 Sep 2010 HIV prevalence is over 33 million worldwide with 68% of AIDS sufferers residing in sub-Saharan Africa (1). Currently the available HIV prevention tools are feasible but women cannot insist on these preventive measures due to social, cultural ... Read more
Expression of Rabies antibodies in tobacco and maize

Author: Zungu, N ; Lotter, T ; Dube, N ; Chakauya, E ; Lezar, S ; Chikwamba, Rachel K Date: Jan 2008 Rabies, an important disease in Asia and Africa, is an acute viral disease of the central nervous system that affects humans and other mammals. Upon bites or contact with rabid animals patients are immediately immunized with antibodies followed ... Read more
GMOs in Africa: opportunities and challenges in South Africa

Author: Bothma, G ; Mashaba, C ; Mkonza, N ; Chakauya, E ; Chikwamba, Rachel K Date: Aug 2010 Genetically modified organisms are expected to have a large impact on the ability of humanity to feed, fuel and heal itself in light of the growing global change, and adverse geo-climatic conditions anticipated as a result of climate change. ... Read more
Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum

Author: Mehlo, L ; Mbambo, Z ; Bado, S ; Lin, J ; Moagi, SM ; Buthelezi, Sindisiwe G ; Stoychev, Stoyan H ; Chikwamba, Rachel K Date: Sep 2013 Physical and biochemical analysis of protein polymorphisms in seed storage proteins of a mutant population of sorghum revealed a mutant with redirected accumulation of kafirin proteins in the germ. The change in storage proteins was accompanied ... Read more
Issues on global food security

Author: Chikwamba, Rachel K Date: 29 Sep 2006 The presentation outlines the global status of food security and implications for meeting the Millennium Development Goals targets, with particular focus on Sub-Saharan Africa; trends in hunger, food availability, food distribution, consumption; ... Read more
Landscape and opportunities for active pharmaceutical ingredient manufacturing in developing African economies

Author: Riley, DL ; Strydom, I ; Chikwamba, Rachel K ; Panayides, Jenny-Lee Date: 2019 Africa is one of the world's fastest growing economies, with South Africa having the fifth highest worldwide pharmaceutical expenditure per capita. In recent years, several companies have considered regional pharmaceutical production but have ... Read more
The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells

Author: Alexandre, Kabamba B ; Gray, ES ; Mufhandu, Hazel T ; McMahon, JB ; Chakauya, E ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Feb 2012 It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) ... Read more
Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN

Author: Alexandre, Kabamba B ; Moore, PL ; Nonyane, M ; Gray, ES ; Ranchobe, N ; Chakauya, E ; McMahon, JB ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Nov 2013 We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured ... Read more
Novel platforms for essential biomolecules: expression of antibodies in plants

Author: Chikwamba, Rachel K ; Lotter, T ; Ramesaar, K Date: 28 Feb 2006 The demand for health products such as protein therapeutics and sub-unit vaccines to control infectious diseases far outweighs the current global production capacity of these molecules. These products are currently being produced in conventional ... Read more
Physiological responses of selected African sorghum landraces to progressive water stress and re-watering

Author: Devnarain, N ; Crampton, BG ; Chikwamba, Rachel K ; Becker, JVW ; O'Kennedy, Maretha M Date: Mar 2016 Sorghum is particularly drought tolerant compared with other cereal crops and is favoured for subsistence farming in water scarce regions of the world. This study was conducted to identify South African sorghum landraces with superior drought ... Read more
Plant made anti-HIV microbicides: a field of opportunity

Author: Lotter-Stark, HCT ; Rybick, EP ; Chikwamba, Rachel K Date: Nov 2012 HIV remains a global burden and without an effective vaccine, it is crucial to develop microbicides to halt the initial transmission of the virus. Several microbicides have been researched with various levels of success. Amongst these ... Read more
Plant-based production of highly potent anti-HIV antibodies with engineered posttranslational modifications

Author: Singh, Advaita Acarya ; Pooe, O ; Kwezi, Lusisizwe ; Lotter-Stark, T ; Stoychev, Stoyan H ; Alexandra, Kabamba B ; Gerber, Isak B ; Bhiman, JN ; Vorster, J ; Pauly, M ; Zeitlin, L ; Whaley, K ; Mach, L ; Steinkellner, H ; Morris, L ; Tsekoa, Tsepo L ; Chikwamba, Rachel K Date: Apr 2020 Broadly neutralising antibodies (bNAbs) against human immunodeficiency virus type 1 (HIV-1), such as CAP256-VRC26 are being developed for HIV prevention and treatment. These Abs carry a unique but crucial post-translational modification (PTM), ... Read more

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