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Browsing by Author "Morris, L"

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Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site

Author: Alexandre, Kabamba B ; Gray, ES ; Pantophlet, R ; Moore, PL ; McMahon, JB ; Chakauya, E ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Sep 2011 The glycans on HIV-1 gp120 play an important role in shielding neutralization sensitive epitopes from antibody recognition. They also serve as targets for lectins that bind mannose-rich glycans. In this study we investigated the interaction ... Read more
Binding of the mannose-specific lectin, Griffithsin, to HIV-1 gp120 exposes the CD4-binding site

Author: Alexandre, Kabamba B ; Gray, ES ; Pantophlet, R ; Moore, PL ; McMahon, JB ; Chakauya, E ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Sep 2011 The glycans on HIV-1 gp120 play an important role in shielding neutralization-sensitive epitopes from antibody recognition. They also serve as targets for lectins that bind mannose-rich glycans. In this study, the authors investigated the ... Read more
Development of aptamer based HIV-1 entry inhibitor prophylactic drugs

Author: London, G ; Mufhandu, Hazel T ; Sok, D ; Morris, L ; Burton, DR ; Mayosi, B ; Khati, M Date: Aug 2013 AIDS remains a major public health problem globally, especially in Southern Africa where over 6.4 million people are infected by the most prevalent HIV-1 subtype C. To help stop the spread of HIV-1 subtype C, we isolated 2ʹ-F-RNA aptamers ... Read more
Effect of expressing an anti-HIV lectin, Griffithsin, in different plant cellular compartments

Author: Stark, T ; Chakauya, E ; Kabamba, A ; Morris, L ; Rybicki, E ; Chikwamba, Rachel K Date: Aug 2010 Sub-Saharan Africa remains the region most heavily affected by HIV, and the majority of new infections from heterosexual contact are in women. Griffithsin (GRFT), a lectin isolated from the red algae Griffithsia, has shown extreme potency ... Read more
Effect of subcellular targeting on the expression and accumulation of Griffithsin in N. benthamiana

Author: Stark, T ; Chakauya, E ; Kabamba, A ; Morris, L ; Rybicki, E ; Chikwamba, Rachel K Date: 01 Sep 2010 Due to the increasing demand for pharmaceuticals and the high cost of production in traditional fermentor systems, plants are being considered as alternative production platforms for therapeutic molecules. One of the considerations for ... Read more
HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide

Author: Van den Berg, N ; Mbobela, P ; Pillay, P ; London, G ; Morris, L ; Maharaj, V ; Khati, M Date: Feb 2011 A natural compound isolated from extracts of an indigenous plant in the Eastern Cape, South Africa, indicates neutralisation activity against HIV-1 pseudoviruses. This natural compound can potentially be used in a microbicide as an alternative ... Read more
HIV-1 subtype C primary isolates exhibit high sensitivity to an anti-gp120 RNA aptamer

Author: Mufhandu, Hazel T ; Alexandre, Kabamba B ; Gray, ES ; Morris, L ; Khati, M Date: Sep 2012 Globally, HIV-1 subtype C is the most prevalent subtype, yet most antiretroviral drugs are developed against subtype B. UCLA1 RNA aptamer, which we previously showed neutralizes HIV-1 subtype C Env-pseudotyped viruses was examined for ... Read more
HIV-1 subtype C unproductively infects human cardiomyocytes in vitro and induces apoptosis mitigated by an anti-Gp120 aptamer

Author: Rangel Lopes de Campos, W ; Chirwa, N ; London, G ; Rotherham, LS ; Morris, L ; Mayosi, BM ; Khati, M Date: Oct 2014 HIV-associated cardiomyopathy (HIVCM) is of clinical concern in developing countries because of a high HIV-1 prevalence, especially subtype C, and limited access to highly active antiretroviral therapy (HAART). For these reasons, we investigated ... Read more
The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells

Author: Alexandre, Kabamba B ; Gray, ES ; Mufhandu, Hazel T ; McMahon, JB ; Chakauya, E ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Feb 2012 It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) ... Read more
Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN

Author: Alexandre, Kabamba B ; Moore, PL ; Nonyane, M ; Gray, ES ; Ranchobe, N ; Chakauya, E ; McMahon, JB ; O'Keefe, BR ; Chikwamba, Rachel K ; Morris, L Date: Nov 2013 We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured ... Read more
Neutralization of several adult and paediatric HIV-1 subtype C isolates using a shortened synthetic derivative of gp120 binding aptamer called UCLA1.

Author: Mufhandu, Hazel T ; Madiga, M ; Gray, E ; Rotherham, L ; Khoza, T ; Morris, L ; Khati, M Date: Jul 2009 This paper present a chemically synthesised derivative of the B40 parental aptamer, called UCLA1 (Cohen et al., 2008), was used for neutralization of endemic subtype C clinical isolates of HIV-1 from adult and paediatric patients and subtype ... Read more
Plant-based production of highly potent anti-HIV antibodies with engineered posttranslational modifications

Author: Singh, Advaita Acarya ; Pooe, O ; Kwezi, Lusisizwe ; Lotter-Stark, T ; Stoychev, Stoyan H ; Alexandra, Kabamba B ; Gerber, Isak B ; Bhiman, JN ; Vorster, J ; Pauly, M ; Zeitlin, L ; Whaley, K ; Mach, L ; Steinkellner, H ; Morris, L ; Tsekoa, Tsepo L ; Chikwamba, Rachel K Date: Apr 2020 Broadly neutralising antibodies (bNAbs) against human immunodeficiency virus type 1 (HIV-1), such as CAP256-VRC26 are being developed for HIV prevention and treatment. These Abs carry a unique but crucial post-translational modification (PTM), ... Read more
UCLA1 aptamer inhibition of human immunodeficiency virus type 1 subtype C primary isolates in macrophages and selection of resistance

Author: Mufhandu, Hazel T ; Alexandre, Kabamba B ; Gray, ES ; Morris, L ; Khati, M Date: Sep 2016 We have previously shown that the aptamer, UCLA1, is able to inhibit HIV-1 replication in peripheral blood mononuclear cells (PBMCs) by binding to residues in gp120. In this study we examined whether UCLA1 was effective against HIV-1 subtype ... Read more
UCLA1, a synthetic derivative of a gp120 RNA aptamer, inhibits entry of human immunodeficiency virus type 1 subtype C

Author: Mufhandu, Hazel T ; Gray, ES ; Madiga, MC ; Tumba, N ; Alexandre, Kabamba B ; Khoza, T ; Wibmer, CK ; Moore, PL ; Morris, L ; Khati, M Date: May 2012 Entry of human immunodeficiency virus type 1 (HIV-1) into cells is mediated by the virion surface envelope (Env) glycoproteins, making it a desirable target for antiretroviral entry inhibitors. We previously isolated a family of gp120 binding ... Read more

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Browsing by Author "Morris, L"

Browsing by Author "Morris, L"

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