ResearchSpace
→
Browsing by Author

Browsing by Author "Jordaan, J"

Now showing items 1-11 of 11

Advances in enzyme immobilisation

Author: Brady, D ; Jordaan, J Date: 10 Jul 2009 Improvements in current carrier-based immobilisation strategies have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit ... Read more
Application of biocatalysis in synthetic chemistry

Author: Brady, D ; Molefe, M ; Steenkamp, Lucia H ; Parkinson, C ; Jordaan, J ; Visser, Daniel F ; Simpson, C ; Chhiba, Varsha P ; Mathiba, K Date: Nov 2006 It was found that the nitrile hydratases in microorganisms isolated from around the world had infra-species amino acid sequence differences, and this provided an explanation for the variability of nitrile substrate usage within the species. ... Read more
Design and fabrication of a micro fluidic circuit for the separation of micron sized particles

Author: Khumalo, F ; Jordaan, J ; Abrahamse, H ; Land, K ; Potgieter, S ; Sparrow, R Date: Jul 2009 The development of a micro fluidic circuit for the separation of micro particles is being investigated. There are a wide range of available separation techniques such as acoustic, laminar flow, split flow, optical trapping and centrifugal ... Read more
Evaluation of novel supports for selective and efficient enrichment of phosphorylated peptides

Author: Bensadek, D ; Chen, W ; Jordaan, J ; Gerber, I ; Lamont, D Date: Jun 2011 A series of novel emulsion based supports MagReSynTM TiO2 (A-D) were evaluated using 32P labelled phosphopeptides and nano LC-MS/MS. The performance of MagReSynTM TiO2 (D) was found to be significantly better in terms of selectivity and ... Read more
Immobilisation and characterisation of biocatalytic co-factor recycling enzymes, glucose dehydrogenase and NADH oxidase, on aldehyde functional ReSynTM polymer microspheres

Author: Twala, BV ; Sewell, BT ; Jordaan, J Date: Mar 2012 The use of enzymes in industrial applications is limited by their instability, cost and difficulty in their recovery and re-use. Immobilisation is a technique which has been shown to alleviate these limitations in biocatalysis. Here we describe ... Read more
Immobilisation and characterisation of glucose dehydrogenase immobilised on ReSyn: a proprietary polyethylenimine support matrix

Author: Twala, BV ; Sewell, BT ; Jordaan, J Date: Jan 2010 Immobilisation of enzymes is of considerable interest due to the advantages over soluble enzymes, including improved stability and recovery. Glucose Dehydrogenase (GDH) is an important biocatalytic enzyme due to is ability to recycle the ... Read more
Isolation, purification and characterization of a novel glucose oxidase from Penicillium sp. CBS 120262 optimally active at neutral pH

Author: Simpson, C ; Jordaan, J ; Whiteley, C ; Gardiner, NS Date: Feb 2007 A novel glucose oxidase (GOX), a Xavoenzyme, from Penicillium sp. was isolated, purified and partially characterised. Maximum activities of 1.08Umg‾1dry weight intracellular and 6.9Uml‾1 extracellular GOX were obtained. Isoelectric focussing ... Read more
Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling

Author: Koenig, C ; Martinez-Val, A ; Naicker, Previn ; Stoychev, S ; Jordaan, J ; Olsen, JV Date: Sep 2023 Tandem mass tags data-dependent acquisition (TMT-DDA) as well as data-independent acquisition-based label-free quantification (LFQ-DIA) have become the leading workflows to achieve deep proteome and phosphoproteome profiles. We present a ... Read more
Spectrophotometric activity microassay for pure and recombinant cytochrome P450-type nitric oxide reductase

Author: Garny, S ; Verschoor, J ; Gardiner, N ; Jordaan, J Date: Feb 2014 Nitric oxide reductase (NOR) of the P450 oxidoreductase family accepts electrons directly from its cofactor, NADH, to reduce two nitric oxide (NO) molecules to one nitrous oxide molecule and water. The enzyme plays a key role in removal of ... Read more
Spherezymes: A novel structured self-immobilisation enzyme technology

Author: Brady, D ; Jordaan, J ; Simpson, C ; Chetty, A ; Arumugam, C ; Moolman, FS Date: 31 Jan 2008 Enzymes have found extensive and growing application in the field of chemical organic synthesis and resolution of chiral intermediates. In order to stabilise the enzymes and to facilitate their recovery and recycle, they are frequently ... Read more
Zirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics

Author: Diez, IA ; Govender, Ireshyn S ; Naicker, Previn ; Stoychev, Stoyan H ; Jordaan, J ; Jensen, ON Date: Apr 2020 Phosphopeptide enrichment is an essential step in large-scale, quantitative phosphoproteomics studies by mass spectrometry. Several phosphopeptide affinity enrichment techniques exist, such as Immobilized Metal ion Affinity Chromatography ... Read more

Now showing items 1-11 of 11

Browse

All of ResearchSpace

Quick Links

Legislation and compliance

General Enquiries

Tel: + 27 12 841 2911
Email: callcentre@csir.co.za

Physical Address
Meiring Naudé Road
Brummeria
Pretoria
South Africa

Postal Address
PO Box 395
Pretoria 0001
South Africa

Social Connect

Resources on this site are free to download and reuse according to associated licensing provision. Please read the terms and conditions of usage of each resource.

Browsing by Author "Jordaan, J"

Browsing by Author "Jordaan, J"

Browse

All of ResearchSpace

Quick Links

Legislation and compliance

General Enquiries

Social Connect