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Browsing by Author "Dubery, IA"

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Ambrafuran (AmbroxTM) synthesis from natural plant product precursors

Author: Ncube, EN ; Steenkamp, Lucia H ; Dubery, IA Date: Aug 2020 Ambergris, an excretion product of sperm whales, has been a valued agent in the formulation of perfumes. The composition of ambergris consists of two major components: 40–46% cholestanol type steroids and approximately 25–45% of a triterpenoid ... Read more
Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacum tissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method

Author: Ncube, EN ; Mhlongo, MI ; Piater, LA ; Steenkamp, PA ; Dubery, IA ; Madala, NE Date: Nov 2014 Chlorogenic acids (CGAs) are a class of phytochemicals that are formed as esters between different derivatives of cinnamic acid and quinic acid molecules. In plants, accumulation of these compounds has been linked to several physiological ... Read more
Bean polygalacturonase inhibitor protein-1 (PGIP-1) inhibits polygalacturonases from Stenocarpella maydis

Author: Berger, DK ; Oelofse, D ; Arendse, MS ; Du Plessis, E ; Dubery, IA Date: Jul 2000 Stenocarpella maydis, a fungal pathogen of maize, produced polygalacturonases (PGs) when grown on pectin or maize cell walls. An extract from bean (Phaseolus vulgaris L.) which contained an active inhibitor of Aspergillus niger PG, also ... Read more
Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions

Author: Madala, NE ; Steenkamp, PA ; Piater, LA ; Dubery, IA Date: Jul 2012 Nicotiana tabacum cell suspensions, 2g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing 40-hexopyranosyloxy-30-methoxyisonitrosoacetophenone ... Read more
Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation

Author: Ncube, EN ; Steenkamp, Paul A ; Madala, NE ; Dubery, IA Date: Jul 2016 Exogenous application of synthetic and natural elicitors of plant defence has been shown to result in mass production of secondary metabolites with nutraceuticals properties in cultured cells. In particular, salicylic acid (SA) treatment has ... Read more
Collision energy alteration during mass spectrometric acquisition is essential to ensure unbiased metabolomic analysis

Author: Madala, NE ; Steenkamp, PA ; Piater, LA ; Dubery, IA Date: Aug 2012 Metabolomics entails identification and quantification of all metabolites within a biological system with a given physiological status; as such, it should be unbiased. A variety of techniques are used to measure the metabolite content of ... Read more
Conversation on data mining strategies in LC-MS untargeted metabolomics: pre-processing and pre-treatment steps

Author: Tugizimana, F ; Steenkamp, Paul A ; Piater, LA ; Dubery, IA Date: Nov 2016 Untargeted metabolomic studies generate information-rich, high-dimensional, and complex datasets that remain challenging to handle and fully exploit. Despite the remarkable progress in the development of tools and algorithms, the “exhaustive” ... Read more c b
Effect of geometrical isomerism of 3,5-dicaffeoylquinic acid on its binding affinity to HIV-integrase enzyme: a molecular docking study

Author: Makola, MM ; Dubery, IA ; Koorsen, G ; Steenkamp, Paul A ; Kabanda, MM ; Du Preez, LL ; Madala, NE Date: 2016 A potent plant-derived HIV-1 inhibitor, 3,5-dicaffeoylquinic acid (diCQA), has been shown to undergo isomerisation upon UV exposure where the naturally occurring, 3(suptrans), 5(suptrans)-diCQA isomer gives rise to the 3(supcis), 5(suptrans)-diCQA, ... Read more c b
Ergosterol-induced sesquiterpenoid synthesis in tobacco Cells

Author: Tugizimana, F ; Steenkamp, PA ; Piater, LA ; Dubery, IA Date: Feb 2012 Plants have the ability to continuously respond to microbial signals in their environment. One of these stimuli is a steroid from fungal membranes, ergosterol, which does not occur in plants, but acts as a pathogen-associated molecular pattern ... Read more
Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger

Author: Ncube, EN ; Mathiba, Kgama ; Steenkamp, Lucia H ; Dubery, IA Date: Oct 2021 Hyphozyma roseoniger is a filamentous yeast used for its biocatalytic ability to convert sclareol, a plant diterpenoid to ambradiol, an intermediate to ambrafuran, which is a sought-after fixative in the perfume industry. Metabolite profiling ... Read more
Influence of the geometric isomers on the radical scavenging properties of 3,5-dica®eoylquinic acid: A DFT study in vacuo and in solution

Author: Makola, MM ; Madala, NE ; Dubery, IA ; Steenkamp, Paul A ; Kabanda, MM Date: Sep 2016 3,5-Dica®eoylquinic acid (diCQA) is a part of the chlorogenic acid group of compounds, largely isolated from food sources and possessing potent antioxidant activity. Only the trans–trans isomer exists in nature, however, abiotic stresses, ... Read more
The Lipopolysaccharide-induced metabolome signature in Arabidopsis thaliana reveals dynamic reprogramming of Phytoalexin and Phytoanticipin pathways

Author: Finnegan, T ; Steenkamp, PA ; Piater, LA ; Dubery, IA Date: 22 Sep 2016 Lipopolysaccharides (LPSs), as MAMP molecules, trigger the activation of signal transduction pathways involved in defence. Currently, plant metabolomics is providing new dimensions into understanding the intracellular adaptive responses to ... Read more
Lipopolysaccharide-induced metabolome signature in Arabidopsis thaliana reveals dynamic reprogramming of Phytoalexin and Phytoanticipin pathways

Author: Finnegan, T ; Steenkamp, Paul A ; Piater, LA ; Dubery, IA Date: Sep 2016 Lipopolysaccharides (LPSs), as MAMP molecules, trigger the activation of signal transduction pathways involved in defence. Currently, plant metabolomics is providing new dimensions into understanding the intracellular adaptive responses to ... Read more c b
Metabolomic analysis of isonitrosoacetophenone-induced perturbations in phenolic metabolism of Nicotiana tabacum cells

Author: Madala, NE ; Steenkamp, PA ; Piater, LA ; Dubery, IA Date: Oct 2013 Plants have developed biochemical and molecular responses to adapt to different stress environments. One of the characteristics of the multi-component defence response is the production of defence-related metabolites. Plant defences can be ... Read more
Metabolomic analysis of methyl jasmonate-Induced triterpenoid production in the medicinal herb Centella asiatica (L.) Urban

Author: James, TJ ; Tugizimana, F ; Steenkamp, PA ; Dubery, IA Date: Apr 2013 Centella asiatica is an important source of biologically active pentacyclic triterpenoids. The enhancement of the biosynthesis of the centellosides by manipulation of associated metabolic pathways is receiving much attention. Jasmonates play ... Read more
Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid

Author: Mhlongo, MI ; Piater, LA ; Steenkamp, Paul A ; Madala, NE ; Dubery, IA Date: Jan 2015 Previous studies suggest that only transisomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated tobacco leaves exposed to ... Read more
Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells

Author: Tugizimana, F ; Steenkamp, PA ; Piater, LA ; Dubery, IA Date: Jan 2014 Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used to investigate the effect of the fungal sterol, ... Read more
Perturbation of pharmacologically relevant polyphenolic compounds in Moringa oleifera against photo-oxidative damages imposed by gamma radiation

Author: Ramabulana, T ; Mavunda, RD ; Steenkamp, Paul A ; Piater, LA ; Dubery, IA ; Madala, NE Date: Mar 2016 Oxidative stress is a physiological state associated with almost all biotic and abiotic stresses in plants. This phenomenon occurs due to imbalances which result from the overproduction of reactive oxygen species (ROS). Plants, however, have ... Read more
Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Author: Makola, MM ; Steenkamp, Paul A ; Dubery, IA ; Kabanda, MM ; Madala, NE Date: Mar 2016 Caffeoylquinic acid (CQA) derivatives are a group of structurally diverse phytochemicals that have attracted attention due to their many health benefits. The structural diversity of these molecules is due in part to the presence of regio- and ... Read more
Profiling of altered metabolomic states in Nicotiana tabacum cells induced by priming agents

Author: Mhlongo, MI ; Steenkamp, Paul A ; Piater, LA ; Madala, NE ; Dubery, IA Date: Oct 2016 Metabolomics has developed into a valuable tool for advancing our understanding of plant metabolism. Plant innate immune defenses can be activated and enhanced so that, subsequent to being pre-sensitized, plants are able to launch a stronger ... Read more

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