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Browsing by Author "Brady, D"

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Cell free phosphorylation method to assess the utility of new nucleotides as nucleoside reserve transcriptase inhibitors

Author: Lebea, Phiyani J ; Bode, ML ; Mathiba, K ; Brady, D Date: Jun 2007 The acyclic nucleoside phosphonates such as cidofovir, adefovir and tenofovir have proved to be effective in vitro (cell culture systems) and in vivo (animal models, clinical studies) against a wide variety of DNA virus and retrovirus infections. ... Read more
Characterisation of nitrilase and nitrile hydratase biocatalytic systems

Author: Brady, D ; Beeton, A ; Zeevaart, J ; Kgaje, C ; Van Rantwijk, F ; Sheldon, RA Date: Mar 2004 Bio-catalytic transformations converting aromatic and arylaliphatic nitriles into the analogous related amide or acid were investigated.These studies included synthesis of the beta-substituted nitrile 3-hydroxy-3-phenylpropionitrile, subsequent ... Read more
Characterisation of the nitrile biocatalytic activity of rhodococcus rhodochrous ATCC BAA-870

Author: Frederick, J ; Brady, D ; Dirr, H Date: Oct 2006 A versatile nitrile-degrading bacterium, Rhodococcus rhodochrous ATCC BAA-870, was isolated through enrichment culturing of soil samples from Johannesburg, South Africa. Useful nitrile-metabolising activity of a potential biocatalyst, Rhodococcus ... Read more
Characterisation of two bifunctional cellulase-xylanase enzymes isolated from a bovine rumen metagenome library

Author: Rashamuse, KJ ; Visser, Daniel F ; Hennessy, F ; Kemp, J ; Van der Merwe, MP ; Badenhorst, J ; Ronneburg, T ; Francis-Pope, R ; Brady, D Date: Feb 2013 Ruminant digestive tract microbes hydrolyse plant biomass, and the application of metagenomic techniques can provide good coverage of their glycosyl hydrolase enzymes. A metagenomic library of circa 70,000 fosmids was constructed from bacterial ... Read more
Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
Decision making in the development of a biocatalytic route for resolution of S-naproxen: from screening to scale-up

Author: Steenkamp, Lucia H ; Brady, D Date: Jun 2010 The non-steroidal anti-inflammatory drug naproxen is most effective as the single S-naproxen enantiomer. However, typical synthetic routes to naproxen yield the racemate of both the R and the S stereoisomers. Biocatalysts can be used to resolve ... Read more
Diamination by n-coupling using a commercial laccase

Author: Wellington, Kevin W ; Steenkamp, P ; Brady, D Date: Feb 2010 Nuclear diamination of p-hydrobenzoquinones with aromatic and aliphatic primary amines was catalyzed by a immobilised commercial laccase, Denilite II Base, from Novozymes. The amine and the p-hydrobenzoquinone was reacted under mild conditions ... Read more
Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous

Author: Chhiba, Varsha P ; Mathiba, Kgama ; Van der Westhuyzen, Christiaan W ; Steenkamp, Paul A ; Rashamuse, JK ; Stoychev, Stoyan H ; Bode, ML ; Brady, D Date: Dec 2018 Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase ... Read more
Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870

Author: Chhiba, Varsha P ; Bode, M ; Mathiba, K ; Kwezi, Lusisizwe ; Brady, D Date: Apr 2012 A range of ß-aminonitriles (3-amino-3-phenylpropanenitrile and derivatives) were synthesised by reaction of various benzonitriles with acetonitrile and subsequent reduction of the resulting acrylonitrile products. These compounds were hydrolysed ... Read more
Enzymatic kinetic resolution of Morita-Baylis-Hillman acetates

Author: Juma, WP ; Chhiba, Varsha P ; Brady, D ; Bode, ML Date: Sep 2017 Racemic Morita-Baylis-Hillman adducts derived from the reaction of acrylonitrile with benzaldehyde, cinnamaldehyde and hydrocinnamaldehyde have been successfully resolved by means of enzymatic kinetic resolution. The (+)-alcohol products were ... Read more
Enzymatic stereoselective synthesis of B-amino acids

Author: Chhiba, Varsha P ; Bode, M ; Mathiba, K ; Brady, D Date: Jun 2014 The use of enzymes for the enantioselective synthesis of single enantiomer ß-subsituted compounds is of interest, as this structural motif occurs commonly in compounds of pharmaceutical importance, such as adrenergic agents, antidiabetics, ... Read more
Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine

Author: Visser, Daniel F ; Gordon, GER ; Bode, ML ; Mathiba, K ; Brady, D Date: May 2012 5-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of ... Read more
A feruloyl esterase derived from a leachate metagenome library

Author: Rashamuse, K ; Sanyika, W ; Ronneburg, T ; Brady, D Date: Jan 2012 A feruloyl esterase encoding gene (designated fae6), derived from a leachate metagenomic library, was cloned and the nucleotide sequence of the insert DNA determined. Translational analysis revealed that fae6 consists of a 515 amino acid ... Read more
Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds

Author: Brady, D ; Dube, N ; Petersen, RL Date: Sep 2006 The application of highly substrate-specific catalysts, such as biocatalysts, can reduce the number of synthetic steps required to generate organic compounds. A wide range of bacteria and yeast cultures were enriched on nitriles as the sole ... Read more
Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds

Author: Brady, D ; Dube, N ; Petersen, R Date: 28 Feb 2006 Nitrile substrate profiles were determined for a wide range of microorganisms. Isolated bacteria and yeast were enriched on nitriles as the sole nitrogen source. Aliphatic and aromatic nitriles were investigated, as well as structurally related ... Read more
High yielding cascade enzymatic synthesis of 5-methyluridine using a novel Purine Nucleoside Phosphorylase, from Bacillus halodurans Alk36

Author: Vissser, D ; Hennessy, F ; Rashamuse, K ; Van Zyl, P ; Gordon, G ; Mathiba, K ; Bode, M ; Pletschke, B ; Brady, D Date: Sep 2010 5-Methyluridine(5-MU) is a non-natural nucleoside that can be used as an intermediate in the synthesis of thymidine, and in the synthesis of nucleoside analogues AZT and stavudine, both of which are used in Highly Active Anti-Retroviral ... Read more
Hydrolysis of nitriles by soil bacteria: variation with soil origin

Author: Rapheeha, OKL ; Roux-van der Merwe, MP ; Badenhorst, J ; Chhiba, Varsha P ; Bode, ML ; Mathiba, Kgama ; Brady, D Date: Mar 2017 The aim of this study was to explore bacterial soil diversity for nitrile biocatalysts, in particular, those for hydrolysis of ß-substituted nitriles, to the corresponding carboxamides and acids that may be incorporated into peptidomimetics. ... Read more
Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation

Author: Visser, Daniel F ; Hennessy, F ; Chhiba, Varsha P ; Lakay, F ; van Heerden, E ; Piater, L ; Moller, C ; Litthauer, D ; Brady, D Date: Oct 2006 Isolating novel enzymes to enhance a commercial process is an area of active research. Biosciences Discovery Biology have established screening methods to isolate genes for novel enzymes from extreme environments. Samples were obtained from ... Read more
An integrated chemo-enzymatic route for preparation of ß-thymidine, a key intermediate in the preparation of antiretrovirals

Author: Gordon, GER ; Bode, ML ; Visser, Daniel F ; Lepuru, MJ ; Zeevaart, JG ; Ragubeer, N ; Ratsaka, M ; Walwyn, DR ; Brady, D Date: Jan 2011 A chemo-enzymatic method for production of ß-thymidine, an intermediate in the synthesis of antiretrovirals, is described. Guanosine and thymine were converted by means of enzymatic transglycosylation to yield 5-methyluridine (5-MU), which ... Read more

Now showing items 6-25 of 36

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